Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.)

Fitri Yuniarti, FitridanWahyu Hidayati, WahyudanLulu Shofaya, Lulu Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.). Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.).

[img]
Preview
Text
MICH-PhD_2018_29.pdf

Download (520kB) | Preview

Abstract

Soursop leaves (Annona muricata L.) is one of the medicinal plants identified as a source of endophytic bacteria producing secondary metabolites. Several studies have reported that secondary metabolite compounds extracted from soursop leaves have inhibitory activity against pathogenic bacteria and fungi and have anticancer activity. This study aims to isolate endophytic bacteria of soursop leaf and identify molecular isolates producing antibacterial metabolites by PCR method. This study began with endophytic bacterial isolation of soursop leaves, followed by screening for antibacterial potency using disc diffusion method and identification of molecular isolates which had the highest antibacterial activity. After isolation, three isolates were obtained: BW-1LM, BW-2LP, and BW-3LK. The result of antibacterial activity test showed that BW-1LM isolate had the highest activity against bacterium of Bacillus subtilis and Salmonella typhi test. Molecular identification was obtained by BW-1LW isolate having 99% similarity level to Bacillus licheniformis DSM 13 strain. The conclusion is soursop leaves contains endophytic bacteria which have antibacterial activity against Bacillus subtilis and Salmonella typhi.

Item Type: Article
Subjects: Fakultas Agama Islam > R Medicine (General)
Fakultas Agama Islam > RS Pharmacy and materia medica
Divisions: Fakultas Keguruan & Ilmu Pendidikan > School of Medicine
Depositing User: Furqon furqon furqon
Date Deposited: 19 Dec 2019 05:50
Last Modified: 19 Dec 2019 05:50
URI: http://repository.uhamka.ac.id/id/eprint/818

Actions (login required)

View Item View Item