Utilization of Bromelain Enzyme from Pineapple Peel Waste on Mouthwash Formula Against Streptococcus mutans

Hanifah, Rahmi dan Ari, Widayanti dan A, Hanif Utilization of Bromelain Enzyme from Pineapple Peel Waste on Mouthwash Formula Against Streptococcus mutans. Utilization of Bromelain Enzyme from Pineapple Peel Waste on Mouthwash Formula Against Streptococcus mutans.

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Abstract

The aim of this research was to apply bromelain enzyme from rough extract of Ananas
comosus (L.) Merr. peel into the mouthwash preparation and investigate the enzyme activity to
inhibit Streptococcus mutans growth.. The research method, bromelain was extracted by means
of a blended pineapple skin, the extract was filtrated and centrifuged to obtain a supernatant.
Enzyme activity was analyzed by spectrophotometer at 275 nm. Mouthwash formula which
involved different concentration of bromelain (20%, 25%, 30%, 35%, 40%, 50%, and 60% (v/v))
was tested the antimicrobial activity by disc diffusion method. The results showed that the formula
with 35% enzyme was an effective in restricting the growth of Streptococcus mutans, with a
relative potential value of 100.40% compared to chlorhexidin 0.1% as possitive control.

Item Type:	Article
Subjects:	H Social Sciences
Divisions:	Fakultas Farmasi dan Sains
Depositing User:	Furqon FFS
Date Deposited:	22 Feb 2019 03:43
Last Modified:	02 Nov 2020 05:48
URI:	http://repository.uhamka.ac.id/id/eprint/642

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